NIH Proteomics Interest Group
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Laboratory of Systems Biology, Cellular Networks Proteomics Unit, NIAID
Principal Investigator: Aleksandra Nita-lazar, Ph.D.

Research in the Proteomics Group focuses on understanding the changes that occur in the cell proteome in response to various stimuli such as cytokines or pathogen-derived molecules, which alter the differentiation state of cells in the immune system or whose production characterizes various disease states. We are especially interested in large-scale absolute quantitative measurements of components in immune cell signaling networks. We will use the resulting large datasets, combined with the data generated by the high-throughput screening efforts of the Molecular and Cell Biology Group and the microarray/next-generation sequencing data from the Genomics and Immunology Groups, to create predictive models of molecular interactions using the software generated by the Computational Biology Group. The predictions of these models will in turn be employed to better understand biological responses at multiple scales of biological organization, including the cell, tissue, and, eventually, whole organism.

Our primary experimental approach to generating the necessary datasets is mass spectrometry, which we will employ together with other proteomic methods using state-of-the art equipment and technologies available in our laboratory and at the National Institutes of Health.

Chemistry and Drug Metabolism Section, NIDA
Principal Investigator: Amina S. Woods, Ph.D.

Epithelial Systems Biology Laboratory, NHLBI
Principal Investigator: Mark A Knepper, MD, PhD

The chief objective of the Knepper Lab is to discover the fundamental mechanisms involved in regulation of the transport of water, urea and ions by the kidney, and to discover how these regulatory processes are altered in various clinically important fluid and electrolyte disorders. A 'systems biology' approach is utilized. A wide variety of technical approaches are used including 1) in vitro perfusion of microdissected renal tubule segments to measure ion and water fluxes; 2) immunochemical approaches to assess the abundance, intracellular distribution, and post-translational modification of physiologically important transporters; 3) biochemical analysis of microdissected renal tubule segments; 4) knockout mouse models; and 5) mass-spectrometry based proteomics methods; 5) mathematical modeling of the kidney as a complex system. The lab heavily exploits the NHLBI Proteomics Core Facility for the mass spectrometry aspects of the studies. The major current areas of emphasis are: 1) discovery of the molecular mechanisms involved in controlling the water channel aquaporin-2 in the renal collecting duct; and 2) application of quantitative mass spectrometry to the discover of vasopressin signaling pathways in the renal collecting duct.

Laboratory of Proteomics and Analytical Technologies, NCI
The Laboratory of Proteomics and Analytical Technologies (LPAT) develops and applies state-of-the-art analytical technologies, primarily mass spectrometry, to advance the understanding of cellular function at the proteomic and metabolomic levels. The LPAT specializes in the identification of individual proteins, characterization of entire protein complexes, and global analysis to discover changes in the proteomes of different samples. The laboratory also specializes in the development of custom assays for quantitation of specific molecules in complex clinical samples. The technologies above are available as core services.

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