NIH Proteomics Interest Group

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ProtIG is an NIH Special Interest Group (SIG) that organizes seminars and workshops in relevant areas of proteomics, including talks on separation and protein identification methods, determination of post-translational modifications, protein-protein interactions, and bioinformatics and data management. A monthly seminar series is held at 10am usually on the first Thursday of each month (always check the Mtgs/Seminars button on this page for these and other PROTIG announced meetings). To receive email announcements of ProtIG events, join the listserv (Join the SIG button on this page)
Please note the location for this seminar is in Building 50.

October ProtIG Seminar
October 3rd, 2014
10:00am - 11:00pm
Building 50, NIH Campus
Room 1227/1233 (first floor lobby)
Alessandra Luchini, Ph.D.
George Mason University


"Protein painting reveals solvent-excluded drug targets hidden within native protein-protein interfaces"

Identifying the contact regions between a protein and its binding partners is essential for creating therapies that block the interaction. Unfortunately, such contact regions are extremely difficult to characterize because they are hidden inside the binding interface. Here we introduce protein painting as a new tool that employs small molecules as molecular paints to tightly coat the surface of protein-protein complexes. The molecular paints, which block trypsin cleavage sites, are excluded from the binding interface. Following mass spectrometry, only peptides hidden in the interface emerge as positive hits, revealing the functional contact regions that are drug targets. We use protein painting to discover contact regions between the three-way interaction of IL1b ligand, the receptor IL1RI and the accessory protein IL1RAcP. We then use this information to create peptides and monoclonal antibodies that block the interaction and abolish IL1b cell signaling. The technology is broadly applicable to discover protein interaction drug targets.


Seminars will be webcast online at http://videocast.nih.gov and available on the
Proteomics Interest Group website http://proteome.nih.gov as an archived presentation unless otherwise noted.


Please remember everyone is welcome to join us for the Fellows Forum Seminar on the 9th of October (in Building 10, Room 3-2550). See the Fellows Forum for more information.

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This site was updated on October 2nd, 2014. Please contact Renee Olano at olanol(at)mail.nih.gov with questions or suggestions.