is an NIH Special Interest Group (SIG)
that organizes seminars and workshops in relevant areas of proteomics,
including talks on separation and protein identification methods,
determination of post-translational modifications, protein-protein
interactions, and bioinformatics and data management. A monthly seminar
series is usually held at 12 pm
on the Second Thursday of each month (always check the Mtgs/Seminars
button on this page for these and other PROTIG announced meetings). To
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|February Special ProtIG Seminar
|Please note the non-standard date.
|Thursday, February 29th, 2024
|1:00 pm - 2:00 pm ET
|NIH Building 50
|Joshua Coon, Ph.D.
|Professor of Biomolecular Chemistry and Chemistry
Director, Laboratory for Biomolecular Mass Spectrometry,
Director, NIGMS National Center for Quantitative Biology of Complex System
Thomas and Margaret Pyle Chair at the Morgridge Institute for Research
University of Wisconsin Madison
|“How Mass Spectrometry can Drive Biological Discovery – from Structure to Systems”
In this presentation I will describe the use of a mass spectrometer to prepare samples for cryo-electron microscopy (cryoEM). Briefly the protein-protein complexes are ionized, vaporized, and pass through a modified Orbitrap MS system where they either have their mass measured or are deposited onto a cryogenically cool TEM grid placed at the rear of system. After being deposited, the landed complexes are coated with a thin film of amorphous ice and then removed from the system and placed into a cryoEM for direct imaging. This method has the potential to resolve many outstanding problems with traditional cryoEM grid preparation (i.e., plunge freezing) including air-water interface issues, preferential orientation, and the ability to precisely control ice thickness. Next, I will provide several examples of high throughput proteome, lipidome, and metabolome measurements and how that can help identify protein function and improve human health. Finally, I will present new MS technology for deep human proteome sequencing for detection of RNA editing and protein phosphorylation.
This seminar will be videocast as a ZoomGov Meeting
Seminars will be webcast online and available on the
Proteomics Interest Group website http://proteome.nih.gov as an archived presentation unless otherwise noted.