NIH Proteomics Interest Group

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ProtIG is an NIH Special Interest Group (SIG) that organizes seminars and workshops in relevant areas of proteomics, including talks on separation and protein identification methods, determination of post-translational modifications, protein-protein interactions, and bioinformatics and data management. A monthly seminar series is usually held at 11am on the first Wednesday of each month (always check the Mtgs/Seminars button on this page for these and other PROTIG announced meetings). To receive email announcements of ProtIG events, join the listserv (Join the SIG button on this page)

February ProtIG Seminar
Please note the time and location
February 1st, 2018
9:30 am - 10:30am
Building 50, NIH Campus
Room 1227/1233 (Front Conference Room)
Shelley Jackson, Ph. D.
Staff Scientist
NIDA-IRP


"Application of MALDI-Ion Mobility Mass Spectrometry in Biological Analysis"

Ion mobility (IM) spectrometry is a robust method that allows for the rapid separation and detection of a wide range of compounds. In this technique, ions in the drift cell obtain an average drift velocity from an electric field based upon their collision cross section (W) or shape, which allows for the separation of molecules depending upon their three-dimensional structure. Combining matrix-assisted laser desorption/ionization (MALDI) mass spectrometry with IM results in the fast sorting of biomolecules in complex mixtures along trend lines. In this 2D analysis of biological families, lipids, peptides, and nucleotides are separated from each other by differences in their ion mobility drift times in a timescale of hundreds of microseconds. Molecular ions of similar chemical type fall along trend lines when plotted in two-dimensional plots of ion mobility drift time as a function of m/z. We have analyzed a wide variety of samples using MALDI-IM MS. This talk will highlight the use of MALDI-IM MS for the analysis of lipids and it advantages in mass spectrometry imaging.



Seminars will be webcast online at http://videocast.nih.gov and available on the
Proteomics Interest Group website http://proteome.nih.gov as an archived presentation unless otherwise noted.



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This site was updated on January 22nd, 2018. Please contact Renee Olano at olanol(at)mail.nih.gov with questions or suggestions.