|
| ||||||
| ||||||
|
|
| ProtIG is an NIH Special Interest Group (SIG) that organizes seminars and workshops in relevant areas of proteomics, including talks on separation and protein identification methods, determination of post-translational modifications, protein-protein interactions, and bioinformatics and data management. A monthly seminar series is usually held at 12 pm on the Second Tuesday of each month (always check the Mtgs/Seminars button on this page for these and other PROTIG announced meetings). To receive email announcements of ProtIG events, join the listserv (Join the SIG button on this page) |
| November ProtIG Seminar |
| Please note the day change for the 2026-2027 seminar season. |
| Tuesday, November 10th, 2026 |
| 11:00 am - 12:00 pm ET |
| NIH Building 35 |
| Room 640 |
| Caroline DeHart, Ph.D |
| Group Leader, Mass Spectrometry NCI RAS Initiatives Cancer Research Technology Program (CRTP) Frederick National Laboratory for Cancer Research National Institutes of Health Frederick, Maryland |
| "Optimizing RAS-MAPK Proteoform Characterization" |
|
Top-down mass spectrometry (TDMS), which entails the analysis of intact and modified protein forms (proteoforms), can precisely localize mutations or post-translational modifications (PTMs) while maintaining the primary protein sequence. This provides unparalleled molecular detail, particularly for the RAS GTPases (HRAS, KRAS4A, KRAS4B, NRAS), which are among the most frequently mutated proteins in human cancer, and which have provided formidable challenges to standard proteomic methods. We have optimized targeted TDMS assays on two instrument platforms to characterize recombinant, exogenous, and endogenous RAS proteoforms, revealing novel PTMs and facilitating the development of new targeted RAS inhibitors. We have also employed intact mass analysis and phosphopeptide parallel reaction monitoring using recombinant BRAF, MEK, 14-3-3, and NF1 proteins to improve our understanding of the RAS-MAPK pathway proteoform landscape and prepare to tackle more challenging targets by TDMS.
|
|
This seminar will be videocast on
|
Seminars will be webcast online and available on the
Proteomics Interest Group website http://proteome.nih.gov as an archived presentation unless otherwise noted. |
This site was updated on November 6th, 2026. Please contact Renee Olano at olanol(at)mail.nih.gov with questions or suggestions.