NIH Proteomics Interest Group

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ProtIG is an NIH Special Interest Group (SIG) that organizes seminars and workshops in relevant areas of proteomics, including talks on separation and protein identification methods, determination of post-translational modifications, protein-protein interactions, and bioinformatics and data management. A monthly seminar series is usually held at 9:30am on the first Thursday of each month (always check the Mtgs/Seminars button on this page for these and other PROTIG announced meetings). To receive email announcements of ProtIG events, join the listserv (Join the SIG button on this page)

February ProtIG Seminar
February 2nd, 2017
9:30am - 10:30am
Building 50, NIH Campus
Room 1328/1334
Michal Fried, Ph.D.
Senior Scientist, Laboratory of Molecular Immunology and Virology
National Institute of Allergy and Infectious Diseases
National Institutes of Health

"Membrane proteome of Plasmodium falciparum parasites collected from children presenting with malaria."

Plasmodium falciparum is the most deadly among malaria parasites that infect humans. In areas of high malaria transmission, young children are most susceptible to disease and high parasite burden, while adults develop immunity that control parasitemia and disease. Proteins expressed on the surface of infected erythrocytes have been described as the targets of immunity to malaria. One of the major protein expressed on the surface of infected erythrocytes belong to a family of variant antigens named Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1). Protective immunity may developed by acquiring antibodies that target conserved or cross-reactive epitopes expressed by PfEMP1. However, antibodies that target exported proteins other than PfEMP1 may also contribute to the development of protective immunity. To identify targets of protective immunity we analyzed the membrane proteome profile of clinical parasite isolates, with the main goal of identifying commonly expressed PfEMP1 and other exported proteins that can be used in serosurveys analysis. One of the major challenges in P. falciparum proteomics studies is identifying PfEMP1s at the protein level due to antigenic variation. To overcome this technical limitation, we developed a pipeline that combines high resolution mass spectrometry with bioinformatics tools including RNAseq on a subset of parasite isolates, de novo sequencing of proteins and sequence alignment to identify PfEMP1 expressed by clinical parasite isolates.

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This site was updated on January 19th, 2017. Please contact Renee Olano at olanol(at) with questions or suggestions.