NIH Proteomics Interest Group

Link to Upcoming Meetings

Link to Meeting Archives

Link to Related Links

Link to NIH Labs

Link Fellows Forum Info

Link to Join SIG

Line









ProtIG is an NIH Special Interest Group (SIG) that organizes seminars and workshops in relevant areas of proteomics, including talks on separation and protein identification methods, determination of post-translational modifications, protein-protein interactions, and bioinformatics and data management. A monthly seminar series is usually held at 12 pm on the Second Thursday of each month (always check the Mtgs/Seminars button on this page for these and other PROTIG announced meetings). To receive email announcements of ProtIG events, join the listserv (Join the SIG button on this page)

November ProtIG Seminar
Thursday, November 14th, 2024
11:00 am - 12:00 pm ET
NIH Building 50
Room 1227/1233
Aleksandra Nita-Lazar, Ph.D.
Senior Investigator, Chief
Functional Cellular Networks
Laboratory of Immune System Biology
National Institute of Allergy and Infectious Diseases
National Institutes of Health


“Signaling mechanisms and protein networks in innate immunity”

Toll-like receptor (TLR) signaling in macrophages is essential for generating effective innate immune responses. Quantitative differences dependent on the dose and timing of the stimulus critically affect cell function and often involve proteins that are not components of widely shared transduction pathways. Mathematical modeling is an important approach to better understand how these signaling networks function in time and space. Wehave successfully modeled the S1P signaling pathway in macrophages using selected reaction monitoring (SRM) to measure the absolute abundance of the pathway proteins. The resulting values became parameters in a computational pathway model. To model the TLR signaling networks, we developed assays for the canonical TLR signaling pathway and related proteins and phosphoproteins and used parallel reaction monitoring (PRM) with heavy-labeled internal peptide standards to quantify protein and phosphorylated protein moleculenumbers per cell in untreated and LPS-stimulated macrophages. The absolute protein abundance values were entered into a model of the TLR pathway developed using Simmune, the rule-based modeling tool with a visual interface. To reach beyond basal level quantification, the TLR signaling network model is tested further and combined with global proteomic approaches to discover biologically important new proteins, protein complexes and PTMs involved in TLR and inflammasome pathways, of which some examples will be given. The protein and PTM levels are quantified in macrophages under diverse, but well-defined conditions (different TLR ligands, whole pathogens, and cells with mutations in specific signaling molecules). These data will allow to parameterize and test the TLR network model under a variety of conditions. Together, the interconnected projects will lead to the better understanding how the immune signaling pathways are regulated and activated during an infection. This research was supported by the Intramural Research Program of NIAID, NIH.





This seminar will be videocast on

NIH Videocast
or
ZoomGov

Please mute your microphone and use the "raising hand" option to indicate a question.



Seminars will be webcast online and available on the
Proteomics Interest Group website http://proteome.nih.gov as an archived presentation unless otherwise noted.



NIH Special Interest Groups home page NIH Home Page

This site was updated on November 13th, 2024. Please contact Renee Olano at olanol(at)mail.nih.gov with questions or suggestions.