A one-day tutorial for NIH scientists and decision makers on the emerging field of proteomics.
The Next Step: Exploring the Proteome
A digital archive of the May 21 Symposium is now
accessible using RealPlayer. Each lecture slide can be viewed
and searched at your command with the speakers' voices synchronized to
Click here to see it!
*The genome of a muticellular organism is constant. In
general, somatic cells contain identical DNA copies.
PROTEOME = PROTEins expressed by the genOME
*Proteomes vary with cell type. Proteins present in one cell type
(for example, hepatocyte) differ from those of another cell type in the
same organism (neuron, fibroblast) although all have the same genome.
*Proteomes vary with cell history and are time dependent.
Proteins present in a newly divided cell differ from those in an older or
stressed or drug-treated cell.
*Proteomes reflect a wide dynamic range of individual protein
concentrations. Some proteins are expressed in thousands of copies
per cell (structural or housekeeping proteins) and others are expressed
transiently as a few copies per cell to control or signal cell events.
*Proteomes reflect active cellular species. Eucaryotic
proteins are post-translationally modified.
Proteome research brings together protein and
analytical chemists, geneticists, molecular biologists and bioinformatics
specialists to capitalize on the availability of human gene sequences and
emerging techniques for the analyses of complex mixtures of
proteins. Symposium speakers will present varying perspectives on
their proteome-based research from clinical medicine to bioinformatics
via application of emerging technologies in protein chips,
electrophoresis and mass spectrometry.
This site was developed by Lewis Pannell. Please contact him by email at
L_Pannell@nih.gov with any problems or suggestions. Thanks!