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| Institute: |
Lab or Branch |
| Bruker Daltonics, Bremen, Germany |
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| Title: |
Sample Preparation for MALDI-TOF/TOF Protein
Identification
with >95 % Success Rates |
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| Authors: |
| D. Suckau, P. Berndt, M. Schuerenberg and
H. Langen |
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| Abstract: |
| The full characterization of human or bacterial
proteomes is a task, which is pursued in industrial proteomics
today at increasing pace. As a rough estimate, MALDI fingerprint
spectra from as many as 50 000 2D–gel spots are
currently required to obtain a basic insight into a proteome.
Unfortunately, in current high-throughput setups typically
less than 60 % of all acquired spectra provide protein
identification. Therefore, there is increasing demand
for high success rates of protein identification and sustained
high throughput in industrial proteomics. Here we present
technologies, which provide significant improvements of
these aspects. Advanced sample preparation protocols and
novel MALDI-TOF/TOF technology are the basis of these
performance increases. All methodological improvements
were tested on the cytosolic extract of the human embryonic
kidney cell line HEK293 as a model proteome. 496 spots
were analyzed in this study. |
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