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| Presenter: |
| Christie Hunter |
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| Institute: |
Lab or Branch |
| Applied Biosystems, Foster City, CA |
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| Title: |
| A Novel Automated PTM Discovery Method
using a Hybrid Linear Quadrupole Ion Trap |
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| Authors: |
| C. Hunter, S. Weintraub, T. Settineri |
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| Abstract: |
| In this study, several phosphopeptide
standards containing S, T and Y phosphorylation were spiked
into a characterized protein digest (500 fmole) and analyzed
by nanoflow LC/MS/MS on the Q TRAPTM System. Several different
concentration levels (20-100 fmole) were used to simulate
a range of phosphorylation levels known to exist in nature.
Automated PTM discovery was performed using information
dependent acquisition (IDA), where a negative ion precursor
ion (PI) scan for m/z 79 was used as a survey scan, followed
by a masscorrected positive ion enhanced resolution (ER)
scan and a positive ion enhanced product ion (EPI) scan.
Neutral loss scans and other PI scans were also evaluated
for phosphopeptide detection and characterization. |
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