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| Institute: |
Lab or Branch |
| Caliper Technologies, Mt. View, CA |
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| Title: |
| Automated LabChip Electrophoresis of DNA
and Proteins |
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| Authors: |
| H. L.Phan, L. Bousse,K. Williams,W. A.
Ausserer |
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| Abstract: |
| Microfluidics-based electrophoresis methods
for analysis of DNA fragments and protein samples are
discussed. The Lab-on-a-Chip system allows sampling from
96- or 384-well plates, electrophoretic separation, fluorescence
detection, and electronic data analysis to be performed
automatically with no user intervention. HaeIII digests
of PhiX 174 DNA are used to qualify the system; accuracy,
reproducibility, resolution, and linearity results are
reported. Separation of PCR-amplified restriction digest
samples of C. elegans is also demonstrated. The LabChip®
electrophoresis system is also suitable for protein analysis,
and ongoing work in this area is discussed. |
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