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| Presenter: |
| Yetrib Hathout |
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| Institute: |
Lab or Branch |
| University of Maryland |
Chemistry and Biochemistry Departments |
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| Title: |
| Highly Altered Protein Expression Profile
in Doxorubicin Resistant MCF-7. |
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| Authors: |
| Y. Hathout, M. Gehrmann, C. Fenselau |
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| Abstract: |
| The protein expression pattern in the cytosol
fraction of doxorubicin resistant MCF-7 was compared to
that of the parental MCF-7 cell line using two dimensional
gel electrophoresis based techniques. Several proteins
were found remarkably altered in their expression in the
doxorubicin resistant cell line. The most striking differences
were proteins that were strongly expressed in this cell
line and not visible in the parental MCF-7 cell line.
These proteins included the ubiquitin carboxyl hydrolase
isoenzyme L1 also known as PGP9.5, glutathione-S-transferase
pi class, nicotinamide N-methyltransferase and interleukin-18
precursor. Conversely, catechol-o-methyltransferase expressed
in the parental cell line was not detected in the doxorubicin
resistant cell line. The alteration of the expression
of these proteins was not observed in MCF-7 cell lines
selected for resistance to etoposide, mitoxantrone or
melphalan. This suggests that breast cancer cells selected
for resistance to different drugs may develop different
mechanisms of resistance. It is possible that this uniquely
altered protein expression might be simply due to the
drug effects on the cells rather than development of resistance.
More studies are needed to correlate protein expression
patterns to development of resistance in cancer cells,
and the use of two dimensional gel electrophoresis seems
an easy tool to screen differential protein expression.
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